Uridine-H³ 및 Leucine-C^(14)에 依한 Mikamycin A가 白鼠顎下腺에 미치는 影響에 關한 二重自己放射法的硏究
INCORPORATION OF URIDINE-H³ AND LEUCINE-C^(14) BY SUBMANDIBULAR GLAND CELLS OF MICE TREATED WITH MI KWMYCIN A
김명국,
소속 상세정보
김명국 ( ) - 서울대학교 치과대학 구강해부학교실
KMID : 0362619890100030163
Abstract
Young adult male mice were used throughout the experiment. Thirty-six mice were paired according to weight and one of the pairs was injected with 0.3ml (20mg/ml) of mikamycin A. The animals were sacrificed on days 1, 4, 8,12,16 and 20. Thirty minutes and one hour prior to sacrifice 5pc/gm of uridine-6-HI (specific activity 5-10c/mM) and 2/,c/gm of 1-leucine-C" (specific activity 250mc/mM) were injected respectively. The organs were fixed in 2% paraformaldehyde in cacodylate buffer and embedded in palodion-paraffin and sectiond at Z stained with toluidine blue 0.
Four sets of slides were made and coated with layers of Kodak NTB-3 liquid emulsion interposed by a layer of celloidin solution.
The present study is to evaluate, by means of quantitative radioautography utilizing double-emulsion and double-isotope techniques, the effects of subleathal doses of mikamycin A on the protein and RNA syntheses in submandibular gland cells.
The results permit the conclusion;
1. Body weight showed a significant decrease during the first 8 days after mikamycin A, while the glandular weight no changed in a all periods.
2. The administration of mikamycin A suppresses the protein and nucleic acid synthesis -in submandibular gland studied.
3. It is concluded that the double-isotope and double-emulsion radioautographic technique can be applied to simultaeneous quantitation of two metabolic aspects in a given cell
population.
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